Exo-Max Exosome Purification Kit
Exosomes and other extracellular vesicles (EVs) are small membrane vesicles containing protein, mRNA, microRNA, DNA, and lipids, which are secreted by various cells and are table in body fluids including blood, urine, saliva etc. These EVs are recognized as biomarkers for many diseases.
Exo-Max Exosome Purification Kit provides an innovative affinity purification method using recombinant protein conjugated magnetic beads, which can specifically capture EVs. This kit can isolate high purity EVs from cell culture medium and body fluids following a 10,000 x g centrifugation. This kit also enables the isolation of intact form of EVs by eluting EVs from magnetic beads with attached reagent. The isolated intact exosomes and other EVs can be used for various applications including electron microscopic analysis, nanoparticle tracking analysis and other protein and molecular analysis such as Western blotting and sequencing.
Exo-Max Exosome Purification Kit provides an innovative affinity purification method using recombinant protein conjugated magnetic beads, which can specifically capture EVs. This kit can isolate high purity EVs from cell culture medium and body fluids following a 10,000 x g centrifugation. This kit also enables the isolation of intact form of EVs by eluting EVs from magnetic beads with attached reagent. The isolated intact exosomes and other EVs can be used for various applications including electron microscopic analysis, nanoparticle tracking analysis and other protein and molecular analysis such as Western blotting and sequencing.
Kit Component:
Note: Exosome Purification Beads is 1:1 suspension in Phosphate Buffered Saline, pH 7.4, containing 0.05% sodium azide as a preservative.
Product Capacity:
The binding and elution capacity of 20 μL Exosome Purification Beads are commonly equal to 10-200 μg of exosomes or EVs.
Materials and reagent required but not provided:
⚫ Micro-centrifuge capable of <10,000 x g
⚫ 1.5 mL Centrifuge tubes
⚫ 15 mL Centrifuge tubes
⚫ 50 mL Centrifuge tubes
⚫ Vortex mixer
⚫ Micro pipette
⚫ Pipette tip
⚫ End-over-end rotator
⚫ Syringe Filter Unit, 0.22 μm, PVDF
⚫ Ultrafiltration unit (e.g. Cut-off 100 K of Vivaspin or Amicon® Ultra Centrifugal Filter Units)
⚫ Magnetic stand
⚫ TBS buffer
Storage:
2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice
| Reagent | Quantity for 10 rxns |
| Exo-Max Exosome Purification Beads | 0.2 mL X 1 vial |
| Binding Buffer | 10 mL X 1 vial |
| Washing Buffer | 20 mL X 1 vial |
| Elution buffer | 1 mL X 2 vial |
| Exo-Cryo Media | 0.5 mL X 1 vial |
Product Capacity:
The binding and elution capacity of 20 μL Exosome Purification Beads are commonly equal to 10-200 μg of exosomes or EVs.
Materials and reagent required but not provided:
⚫ Micro-centrifuge capable of <10,000 x g
⚫ 1.5 mL Centrifuge tubes
⚫ 15 mL Centrifuge tubes
⚫ 50 mL Centrifuge tubes
⚫ Vortex mixer
⚫ Micro pipette
⚫ Pipette tip
⚫ End-over-end rotator
⚫ Syringe Filter Unit, 0.22 μm, PVDF
⚫ Ultrafiltration unit (e.g. Cut-off 100 K of Vivaspin or Amicon® Ultra Centrifugal Filter Units)
⚫ Magnetic stand
⚫ TBS buffer
Storage:
2 °C to 8 °C. DO NOT FREEZE.
Shipping Conditions:
Blue ice